What is Regenerative Plasma Concentrate (RPC)?
Regenerative Plasma Concentrate (RPC) is an advanced form of bioactive plasma therapy developed by Dr. John Hughes to enhance tissue repair, reduce inflammation, and accelerate healing. This ozonated regenerative plasma treatment combines concentrated platelet growth factors (from both platelet-rich and platelet-poor plasma), autologous stem cells, essential nutrients, and therapeutic peptides. Harvested from the patient’s own blood through a simple draw, RPC is prepared and infused to stimulate cellular regeneration, support recovery from injuries, and optimize overall brain function.
What is Regenerative Plasma Concentrate (RPC) used to Treat?
- Traumatic Brain Injury (TBI)
- Concussion
- Post-Traumatic Stress Disorder (PTSD)
- Neurological Decline
- Alzheimer’s Disease
- Memory Issues
- As well as orthopoedic conditions
“In June 2017, I went in for my second intranasal stem cell procedure and by August I felt well enough that I started saying yes again to facilitating events and speaking gigs. I also experienced relief from anxiety. Within 8-12 weeks post procedure I experienced a noticeable jump in my healing. TBI Therapy has turned me into a TBI THRIVER, not just a survivor. I’m happy. I enjoy life again, can travel and am doing work in the world that’s more aligned with myself than ever.”
Intranasal RPC Procedure
At our medical clinics in Aspen and Basalt, Colorado, we offer Regenerative Plasma Concentrate (RPC)—a form of advanced bioactive plasma therapy designed to support neurological and cellular regeneration. The process begins with a simple blood draw, from which the patient’s own plasma is extracted and refined.
During RPC preparation, patients receive a customized nutrient IV infusion to enhance mitochondrial health, optimize recovery, and improve overall treatment efficacy.
Once activated, the RPC is administered using Dr. John Hughes’ patented intranasal catheter, which delivers the plasma cocktail to the upper nasal cavity for direct access to the brain’s regenerative pathways. Patients recline comfortably with the head positioned for optimal absorption while the RPC integrates into targeted neural tissues. The full procedure lasts approximately two hours, including a 15-minute infusion period.
Following treatment, patients typically resume normal activities the same day. Many report a sense of calm, clarity, or mild euphoria within hours, along with improved cognitive function over time. The most noticeable regenerative results generally emerge within one to three months.
For enhanced outcomes, RPC can be paired with Hyperbaric Oxygen Therapy (HBOT) sessions before and after treatment to further stimulate oxygen delivery, tissue repair, and brain recovery.
Individual responses vary; no specific results are guaranteed.
What makes RPC effective?
Concentrated Platelet Poor Plasma
Platelet-Poor Plasma (PPP), often considered a byproduct of platelet-rich plasma (PRP) production, contains bioactive factors, cytokines, and proteins that can support tissue healing and, when applied to neural environments, contribute to neuroprotection, anti-inflammatory responses, and potential neuronal repair. While less studied than PRP for nerve regeneration, PPP still holds potential for therapeutic applications.
- Neuroprotection: Similar to PRP, PPP contains proteins that can support the survival of neurons.
- Anti-inflammatory Effects: PPP has been shown to have anti-inflammatory and, in some contexts, antioxidant properties, which are beneficial for mitigating damage in neurological disorders.
- Tissue Regeneration: The growth factors and nutrients present in PPP (similar to those in PRP but at lower concentrations) may help support the microenvironment for damaged nerve tissues.
- Neurorestoration: The proteins in PPP may aid in neurorestorative processes, contributing to potential recovery in neurodegenerative conditions or traumatic brain injuries.
While research specifically targeting the brain with only PPP is limited, its composition of growth factors and cytokines suggests its potential as a regenerative agent to support neuron health.
https://pmc.ncbi.nlm.nih.gov/articles/PMC9243829/
Concentrating platelet-poor plasma (PPP) and administering it to the brain generally introduces a high concentration of plasma proteins—such as fibrinogen, prothrombin, and fibronectin—which are known to act as sealing or healing agents in damaged tissues. While Platelet-Rich Plasma (PRP) is more commonly studied for neuroregeneration, concentrated PPP is utilized in regenerative medicine to promote tissue remodeling and reduce inflammation, often serving as a controlled, non-cellular therapeutic component.
When applied to the brain or nervous system, research indicates the following potential effects:
- Promotes Healing and Reduces Scarring: Unlike PRP, which can sometimes increase fibrosis (scarring) in certain muscle tissues, PPP is thought to encourage the growth of functional tissue without increasing the risk of, and sometimes reducing, detrimental scaring, making it potentially useful for delicate neural environments.
- Modulation of Inflammation: PPP provides a rich source of plasma-derived factors that can reduce neuroinflammation, which is a major factor in neurodegenerative diseases and brain injuries.
- Supportive Microenvironment: PPP acts as a scaffold-like substance that can stabilize injured areas, improving the overall metabolic and structural environment for neural repair.
- Comparison to Platelet Lysate/PRP: While platelet lysates (from concentrated platelets) are more effective at driving active neural stem cell proliferation and regeneration via growth factors (BDNF, VEGF), concentrated PPP provides the structural and biochemical support (fibrinogen) necessary for the initial phases of repair.
In Summary: Concentrated PPP acts as a healing agent that aids in remodeling and reducing inflammation, fostering a more stable environment for brain repair compared to untreated or heavily injured states.
Concentrated Platelet Rich Plasma
Concentrated platelet-rich plasma (PRP), which contains a high concentration of growth factors (such as BDNF, VEGF, TGF-), facilitates neuroregeneration by reducing neurotoxicity, mitigating apoptosis (cell death), and reducing neuroinflammation. Studies indicate it promotes axonal regeneration, improves memory function, and enhances synaptic plasticity in, for example, hippocampal neurons.
Key Effects of Concentrated Platelets on Neurons:
- Neuroprotection: Platelet factors (like Brain-Derived Neurotrophic Factor – BDNF) protect neurons from apoptosis, especially in neurodegenerative conditions or acute injuries.
- Neural Regeneration: Promotes axonal outgrowth and neuronal repair by stimulating angiogenesis (vessel growth) and releasing growth factors.
- Synaptic Plasticity Improvement: Enhances neurotransmitter release and improves long-term potentiation (LTP), which is critical for memory and learning, particularly in models of dementia.
- Anti-inflammatory Response: Reduces neuroinflammation by inhibiting microglia activation and modifying the immune response in the brain.
When applied to the brain or nervous system, research indicates the following potential effects:
- Promotes Healing and Reduces Scarring: Unlike PRP, which can sometimes increase fibrosis (scarring) in certain muscle tissues, PPP is thought to encourage the growth of functional tissue without increasing the risk of, and sometimes reducing, detrimental scaring, making it potentially useful for delicate neural environments.
- Modulation of Inflammation: PPP provides a rich source of plasma-derived factors that can reduce neuroinflammation, which is a major factor in neurodegenerative diseases and brain injuries.
- Supportive Microenvironment: PPP acts as a scaffold-like substance that can stabilize injured areas, improving the overall metabolic and structural environment for neural repair.
- Comparison to Platelet Lysate/PRP: While platelet lysates (from concentrated platelets) are more effective at driving active neural stem cell proliferation and regeneration via growth factors (BDNF, VEGF), concentrated PPP provides the structural and biochemical support (fibrinogen) necessary for the initial phases of repair.
In Summary: Concentrated PPP acts as a healing agent that aids in remodeling and reducing inflammation, fostering a more stable environment for brain repair compared to untreated or heavily injured states.
Ozone
Key Effects of Ozone on Platelets:
- Activation and Aggregation: Ozone acts as a stimulant on platelets, promoting their aggregation, especially in the presence of anticoagulants like heparin.
- Release of Growth Factors: Ozone treatment induces a marked increase in the release of platelet-derived factors, such as platelet-derived growth factor (PDGF), transforming growth factor beta1 (TGF-$\beta$1), and interleukin-8 (IL-8).
- Morphological Changes: Microscopic studies show that ozone induces increased surface protrusions and dilation of the open canalicular system, which are indicators of high secretory activity.
- Microparticle Release: Ozone can damage cell membranes, leading to a dose-dependent increase in the release of microparticles.
- Sensitivity: Platelets are highly sensitive to the oxidative stress induced by ozone, sometimes more so than erythrocytes.
- Clinical Utility: When used in PRP, these effects are thought to enhance healing by releasing growth factors from platelet granules.
It is important to note that the effects are often mediated by the formation of transient reactive oxygen species (ROS) and lipid oxidation products. However, the response can be modulated by the type of anticoagulant used; for example, citrate reduces ozone-induced aggregation compared to heparin.
Dextrose
Dextrose solution (delivered IV and IN)
High-concentration dextrose (specifically 25% or higher) activates platelets, causing a significant increase in mean platelet volume (MPV) and a rapid, partial reduction in platelet count. This effect, often studied in regenerative medicine, suggests that hypertonic dextrose induces platelet shape change from discoid to globular, releasing growth factors.
Key findings from research on dextrose and platelets include:
- Platelet Activation: Exposure to high-concentration glucose causes an influx of calcium into platelets, leading to increased MPV.
- Volume Increase: Concentrations of 25% or higher significantly increase MPV, with 50% dextrose showing a pronounced effect.
- Count Reduction: Hypertonic dextrose causes a rapid, initial drop in platelet count (partial lysis).
- Stabilization: After the initial drop, platelet counts tend to stabilize within 15 seconds to 30 minutes.
- Regenerative Role: In musculoskeletal treatments, this reaction is thought to trigger the release of cytokines and growth factors
Magnesium Chloride
Magnesium chloride (low dose)
- Stabilization: Studies indicate that increased magnesium can stabilize platelets and prevent structural changes during cold storage.
Note: While some studies show inhibition, other research indicates that in specific contexts (like citrated blood), magnesium can actually help restore normal platelet function and coagulation, suggesting a dual, context-dependent role.
Based on research studies, here is what 50 mg (or similar, low-concentration additions) of magnesium chloride does to citrated blood:
- Facilitates Platelet Activation: Magnesium helps facilitate platelet aggregation and activation, reversing the inhibitory effect of citrate, especially if the sample has been re-calcified.
NAD+
NAD+ (nicotinamide adenine dinucleotide) maintains blood plasma homeostasis, supporting the activity of sirtuins and other enzymes that regulate growth factors involved in cell proliferation, repair, and metabolism. By boosting NAD+ levels, you help reverse age-related declines in cell signaling, improving tissue homeostasis, mitochondrial quality, and endothelial proliferation.
Key impacts of NAD+ on growth factor regulation include:
- Sirtuin Activation (SIRT1): NAD+ serves as a critical substrate for sirtuins, enzymes that regulate growth-related signaling pathways, including those involved in mitochondrial biogenesis, autophagy, and tissue regeneration.
- Pro-angiogenic and Proliferative Effects: Increased NAD+ promotes the proliferation of endothelial cells, which is essential for blood vessel health.
- Cell Signaling Modulation: NAD+ acts as a metabolic messenger, ensuring proper cellular adaptation to stress by modulating growth-related pathways such as the Akt signaling pathway, which is vital for neural stem/progenitor cell proliferation.
- Stemness and Regeneration: NAD+ depletion contributes to the loss of stemness (regenerative capacity) in tissues, which is restored by raising NAD+ levels.
As we age, plasma NAD+ levels decline, leading to decreased tissue functionality and regeneration. Restoring NAD+ helps maintain optimal signaling for cell survival and growth, effectively acting as a therapeutic agent to counteract age-related decline.
NAD is administered separately, not mixed.
Glutathione
Glutathione is the brain’s primary antioxidant and detoxifier, crucial for protecting neurons against oxidative stress, reducing neuroinflammation, and maintaining neurotransmitter balance. It prevents neurodegeneration by scavenging free radicals, preserving mitochondrial function, and acting as a reservoir for neuronal glutamate, which supports cognitive performance.
Key Functions of Glutathione in the Brain:
- Antioxidant Defense: It acts as the major scavenger of reactive oxygen species (ROS), protecting neurons from damage that leads to neurodegenerative diseases like Alzheimer’s and Parkinson’s.
- Neurotransmitter Modulation: It supports the function of neurotransmitters such as serotonin and dopamine, which are critical for mood and cognition.
- Detoxification: It helps remove toxins and heavy metals from brain cells.
- Cellular Maintenance: It stabilizes cell membranes and regulates DNA synthesis and repair.
- Metabolic Support: It plays a role in energy production, and its depletion is linked to cell death and brain injury.
Key Connections to Brain Health:
- Neuroprotection: Adequate glutathione levels are essential to prevent the neuronal damage that precedes or accompanies cognitive decline and neurodegenerative diseases.
- Role of Astrocytes: Astrocytes are key in producing and supplying glutathione to neighboring neurons, especially during times of oxidative stress.
- Therapeutic Potential: Because of its role in neuroprotection, increasing brain glutathione levels is researched as a strategy to treat stroke, Alzheimer’s, and other neurodegenerative conditions.
Glutathione is administered separately, not mixed.
References
What does Regenerative Plasma Concentrate (RPC) Cost?
The RPC intranasal and IV infusion is offered at $5,500.
For patients seeking a more comprehensive approach, TBI Therapy offers multi-day packages (2-, 3-, and 10-day options) that may include hyperbaric oxygen therapy (HBOT), intranasal insulin, basic blood labs, cognitive and brainwave assessments, and additional supportive therapies tailored to optimize neurological recovery.
Please note that these services are not billed to insurance. However, we can provide a superbill upon request to assist with potential out-of-network reimbursement.