What is PRP?
PRP is an autologous concentration of human platelets to supraphysiologic levels. Platelets are irregularly shaped, nonnucleated cytoplasmic bodies derived from fragmentation of megakaryocyte precursors. PRP is a specialized form of blood plasma that is rich in growth factors, cytokines, and stem cell precursors.
The growth factors from PRP promote cell differentiation and maturation. When combined with a stem cell treatment, the stem cells are like planting the seeds and the PRP is like the soil, water, fertilizer, and sunlight that help the seeds mature and grow.
As part of our patented TBI Therapy Protocol, insulin and PRP infusions can be administered in coordination with another regenerative injection therapy – stem cell therapy – to help accelerate regrowth and rehabilitation in the brain.
Benefits of PRP
- Regrowth of brain collagen (proteins that provide strength and structure)*
- Activation and targeting of stem cells (cellular regeneration and differentiation)*
- Increased angiogenesis (the development of new blood vessels)*
- Decreased inflammation (increasing brain functionality)*
- Reduction of amyloid proteins (which result in memory loss)*
*Results may vary; no guarantee of specific results
At baseline levels, platelets function as a natural reservoir for growth factors, including platelet-derived growth factor (PDGF), epidermal growth factor (EGF), transforming growth factorbeta 1 (TGFβ1), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (FGF), hepatocyte growth factor (HGF), and insulin-like growth factor (IGFI). There is a general consensus in PRP research that the injection of concentrated platelets, once activated, results in an exponential increase in numerous growth factors at the sight of PRP injection.
Intranasal PRP Procedure
PRP is harvested from the patient’s blood by a simple blood draw. During infusion, the patient will lay back on the exam table with their head tilted perpendicular to the ground. After local anesthetic is sprayed into the nose, the PRP combined with insulin and other nutrients is dripped up the nose with a special device. The patient will lie there for another 15 minutes while the PRP fuses into the brain. After this, the patient is free to get up and continue their daily activities.
Why Insulin is Added to Intranasal PRP
Insulin and PRP can access the cerebrospinal fluid (CSF) along the olfactory neurons through the cribriform plate, or they can enter the CNS parenchyma through perivascular channels associated with the olfactory or trigeminal systems. In addition, a slower axonal transport pathway has been identified along which insulin can access the CNS several hours after intranasal administration.
A number of compounds, including insulin and insulin-like growth factorI, have been successfully delivered to the brain or CSF following intranasal administration. Intranasal insulin administration increases CSF insulin levels in humans about 10 minutes after the injection, with peak levels achieved in about 30 minutes. Blood glucose and insulin levels do not change after intranasal administration, demonstrating that the changes in CSF are not due to transport from the nasal cavity to the systemic circulation.
Intranasal insulin administration has facilitated verbal memory in adults with AD and mild cognitive impairment who were not APOEε4 carriers. For memory-impaired ε4− adults, 10, 20, and 40 IU of insulin has improved declarative memory. The cognitive dose-response curves for this group has shown that memory facilitation generally peaks at the 20 IU dose.*
*Results may vary: no guarantee of specific results
How Do Growth Factors in PRP Aid Healing?
- Macrophage activation and angiogenesis
- Fibroblast chemotaxis and proliferative activity
- Enhances collagen synthesis
- Enhances the proliferation of bone cells
IGF1 (Insulin-Like Growth Factor-I):
- Chemotactic for myoblast and fibroblasts and stimulates protein synthesis
- Mediator in growth ad repair of skeletal muscle
- Enhances bone formation by proliferation and differentiation of osteoblasts
TGFp (Transforming Growth Factorβ):
- Enhances the proliferative activity of fibroblasts
- Stimulates biosynthesis of type I collagen and fibronectin
- Induces deposition of bone matrix
- Inhibits osteoclast formation and bone resorption
- Regulation in balance between fibrosis and myocyte regeneration
PDEGF (Platelet-Derived Endothelial Growth Factor):
- Promotes wound healing by stimulating the proliferation of keratinocytes and dermal fibroblasts
PDAF (Platelet-Derived Angiogenic Factor)
- Induces vascularization by stimulating vascular endothelial cells
EGF (Endothelial Growth Factor):
- Cellular proliferation
- Differentiation of epithelial cells
VEGF (Vascular Endothelial Growth Factor)
- Migration and mitosis of endothelial cells
- Creation of blood vessel lumen
- Creation of fenestrations
- Chemotactic for macrophages and granulocytes
- Vasodilation (indirectly by release of nitrous oxide)
HGF (Hepatocyte Growth Factor):
- Stimulates of hepatocyte proliferation and liver tissue regeneration
- Mitogen for endothelial cells